• Product name
  • Description
    Rabbit polyclonal to eIF4A3
  • Host species
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human eIF4A3 (NP_055555.1).

  • Positive control
    • Hela whole cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab115022 was affinity purified using an epitope specific to eIF4A3 immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab115022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the DEAD box helicase family. eIF4A subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization
      Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP-dependent RNA helicase DDX48 antibody
      • ATP-dependent RNA helicase eIF4A-3 antibody
      • DDX48 antibody
      • DEAD box protein 48 antibody
      • eIF-4A-III antibody
      • eIF4A-III antibody
      • EIF4A3 antibody
      • eIF4AIII antibody
      • Eukaryotic initiation factor 4A-III antibody
      • Eukaryotic initiation factor 4A-like NUK-34 antibody
      • Eukaryotic translation initiation factor 4A isoform 3 antibody
      • hNMP 265 antibody
      • IF4A3_HUMAN antibody
      • NMP 265 antibody
      • NMP265 antibody
      • Nuclear matrix protein 265 antibody
      • NUK34 antibody
      see all


    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling eIF4A3 with ab115022 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling eIF4A3 with ab155022 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Detection of eIF4A3 by Western Blot of Immunprecipitate.
      Anti-eIF4A3, at 0.4 µg/ml, staining eIF4A3 Lin Hea whole cell lysate immunoprecipitated using ab115022 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.


    ab115022 has not yet been referenced specifically in any publications.

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