Product nameAnti-eIF4A3 antibody
See all eIF4A3 primary antibodies
DescriptionRabbit polyclonal to eIF4A3
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
Synthetic peptide within Human eIF4A3 aa 125-175. The exact sequence is proprietary. NP_055555.1
Database link: P38919
- IHC-P: Human ovarian carcinoma tissue. Mouse renal carcinoma tissue. WB: HeLa and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 47 kDa.|
FunctionATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
- Information by UniProt
- ATP-dependent RNA helicase DDX48 antibody
- ATP-dependent RNA helicase eIF4A-3 antibody
- DDX48 antibody
All lanes : Anti-eIF4A3 antibody (ab264315) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes
eIF4A3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264315 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264315 at 0.4 µg/ml.
Lane 1: ab264315 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal carcinoma tissue labeling eIF4A3 with ab264315 at 1/200 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling eIF4A3 with ab264315 at 1/200 dilution. Detection: DAB. Different lots used in each panel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264315 has not yet been referenced specifically in any publications.