Overview

  • Product name

    Anti-eIF4A3 antibody [EPR14301(B)]
    See all eIF4A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14301(B)] to eIF4A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human eIF4A3 aa 50-150. The exact sequence is proprietary.
    Database link: P38919

  • Positive control

    • MCF7, HeLa, Raji and HepG2 whole cell lysate (ab7900); MCF7 and HeLa cells; Human prostate tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14301(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100.
ICC/IF 1/500.
IP 1/30.

Target

  • Function

    ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP-dependent RNA helicase DDX48 antibody
    • ATP-dependent RNA helicase eIF4A-3 antibody
    • DDX48 antibody
    • DEAD box protein 48 antibody
    • eIF-4A-III antibody
    • eIF4A-III antibody
    • EIF4A3 antibody
    • eIF4AIII antibody
    • Eukaryotic initiation factor 4A-III antibody
    • Eukaryotic initiation factor 4A-like NUK-34 antibody
    • Eukaryotic translation initiation factor 4A isoform 3 antibody
    • hNMP 265 antibody
    • IF4A3_HUMAN antibody
    • NMP 265 antibody
    • NMP265 antibody
    • Nuclear matrix protein 265 antibody
    • NUK34 antibody
    see all

Images

  • All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution

    Lane 1 : MCF7 cell line with NFDM
    Lane 2 : Hela cell line with NFDM
    Lane 3 : Raji cell line with NFDM
    Lane 4 : HepG2 cell line with NFDM

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.

  • Immunofluorescent analysis of 4% paraformaldehyde Hela cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/200.

  • Flow cytometric analysis of 2% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/160 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Immunoprecipitation of Hela labeling eIF4A3 using ab180573 at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.



    All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/30 dilution

    Lane 1 : Hela cell lysate with NFDM
    Lane 2 : Negative control with NFDM

    Blocking peptides at 5 % per lane.

References

This product has been referenced in:

  • Ren X  et al. Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export. Front Microbiol 10:1625 (2019). Read more (PubMed: 31379779) »
  • Viphakone N  et al. Co-transcriptional Loading of RNA Export Factors Shapes the Human Transcriptome. Mol Cell 75:310-323.e8 (2019). Read more (PubMed: 31104896) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse ESC (CCE))
Total protein in input
6e+006 cells
Immuno-precipitation step
Protein G
Specification
mouse ESC (CCE)

Abcam user community

Verified customer

Submitted Jan 30 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cell line (CCE))
Permeabilization
Yes - 0.25% TritonX-100 solution (dilute in PBS)
Specification
mouse ES cell line (CCE)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 27 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ESC (CCE))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Mouse ESC (CCE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 26 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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