Product nameAnti-eIF4A3 antibody [EPR14301(B)]
See all eIF4A3 primary antibodies
DescriptionRabbit monoclonal [EPR14301(B)] to eIF4A3
Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
Synthetic peptide within Human eIF4A3 aa 50-150. The exact sequence is proprietary.
Database link: P38919
- MCF7, HeLa, Raji and HepG2 whole cell lysate (ab7900); MCF7 and HeLa cells; Human prostate tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab180573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
- Information by UniProt
- ATP-dependent RNA helicase DDX48 antibody
- ATP-dependent RNA helicase eIF4A-3 antibody
- DDX48 antibody
All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution
Lane 1 : MCF7 cell line with NFDM
Lane 2 : Hela cell line with NFDM
Lane 3 : Raji cell line with NFDM
Lane 4 : HepG2 cell line with NFDM
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
All lanes : Goat Anti-rabbit HRP at 1/1000 dilution
Predicted band size: 47 kDa
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.
Immunofluorescent analysis of 4% paraformaldehyde Hela cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/200.
Flow cytometric analysis of 2% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/160 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.
Immunoprecipitation of Hela labeling eIF4A3 using ab180573 at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/30 dilution
Lane 1 : Hela cell lysate with NFDM
Lane 2 : Negative control with NFDM
Blocking peptides at 5 % per lane.
This product has been referenced in:
- Ren X et al. Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export. Front Microbiol 10:1625 (2019). Read more (PubMed: 31379779) »
- Viphakone N et al. Co-transcriptional Loading of RNA Export Factors Shapes the Human Transcriptome. Mol Cell 75:310-323.e8 (2019). Read more (PubMed: 31104896) »