Overview

  • Product name

    Anti-eIF4B antibody [1F5]
    See all eIF4B primary antibodies
  • Description

    Mouse monoclonal [1F5] to eIF4B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant fragment of Human eIF4B expressed in E. Coli.

  • Positive control

    • A549, A431, HepG2, HEK293, Jurkat, K562 and MCF-7 cell lysates. HeLa and NIH3T3 cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR185156-5 are from Tissue Culture Supernatant

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1F5
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function

    Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310046H11Rik antibody
    • AL024095 antibody
    • C85189 antibody
    • EIF 4B antibody
    • eIF-4B antibody
    • EIF4B antibody
    • Eukaryotic initiation factor 4B antibody
    • Eukaryotic translation initiation factor 4B antibody
    • IF4B_HUMAN antibody
    • PRO1843 antibody
    see all

Images

  • All lanes : Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HEK293 cell lysate
    Lane 5 : HeLa cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : K562 cell lysate
    Lane 8 : NIH3T3 cell lysate
    Lane 9 : MCF-7 cell lysate

    Predicted band size: 69 kDa

  • ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.
  • ab129369 at 1/200 dilution staining eIF4B in NIH3T3 cells by immunofluorescence. DNA was stained with DRAQ5 dye (blue) and actin with Alexa 555 phalloidin (red).
  • Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution + eIF4B recombinant fragment (aa 381-585)

    Predicted band size: 69 kDa



    The expected molecular weight of the recombinant fragment is 48 kDa.

References

ab129369 has not yet been referenced specifically in any publications.

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