Key features and details
- Mouse monoclonal [1F5] to eIF4B
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Product nameAnti-eIF4B antibody [1F5]
See all eIF4B primary antibodies
DescriptionMouse monoclonal [1F5] to eIF4B
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Recombinant fragment
Purified recombinant fragment of Human eIF4B expressed in E. Coli.
- A549, A431, HepG2, HEK293, Jurkat, K562 and MCF-7 cell lysates. HeLa and NIH3T3 cells.
This product was changed from ascites to supernatant. Lot no’s high than GR185156-5 are from Tissue Culture Supernatant
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab129369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).|
|Flow Cyt||1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200 - 1/1000.|
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
- Information by UniProt
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
All lanes : Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : K562 cell lysate
Lane 8 : NIH3T3 cell lysate
Lane 9 : MCF-7 cell lysate
Predicted band size: 69 kDa
ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.
ab129369 at 1/200 dilution staining eIF4B in NIH3T3 cells by immunofluorescence. DNA was stained with DRAQ5 dye (blue) and actin with Alexa 555 phalloidin (red).
Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution + eIF4B recombinant fragment (aa 381-585)
Predicted band size: 69 kDa
The expected molecular weight of the recombinant fragment is 48 kDa.
ab129369 has not yet been referenced specifically in any publications.