Anti-eIF4B antibody (ab171820)

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Western blot - Anti-eIF4B antibody (ab171820)

    Key features and details

    • Mouse polyclonal to eIF4B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-eIF4B antibody
      See all eIF4B primary antibodies

    • Description

      Mouse polyclonal to eIF4B

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse

    • Immunogen

      Full length protein corresponding to Human eIF4B aa 1-611.
      Sequence:

      MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGD VSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFL GNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSL LSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWR ARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPCRDMD RYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYED RYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSAS TSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRP RERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENET LNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDT EQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDG GNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDG EDENEGEDYAE


      Database link: AAH98437.1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • eIF4B-transfected 293T cell lysate.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.4
      Constituent: 100% PBS
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab171820 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa.

    Target

    • Function

      Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

    • Sequence similarities

      Contains 1 RRM (RNA recognition motif) domain.

    • Post-translational
      modifications

      Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 2310046H11Rik antibody
      • AL024095 antibody
      • C85189 antibody
      • EIF 4B antibody
      • eIF-4B antibody
      • EIF4B antibody
      • Eukaryotic initiation factor 4B antibody
      • Eukaryotic translation initiation factor 4B antibody
      • IF4B_HUMAN antibody
      • PRO1843 antibody
      see all

    Images

    • Western blot - Anti-eIF4B antibody (ab171820)
      Western blot - Anti-eIF4B antibody (ab171820)
      All lanes : Anti-eIF4B antibody (ab171820) at 1 µg/ml

      Lane 1 : eIF4B-transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa

    References (0)

    Publishing research using ab171820? Please let us know so that we can cite the reference in this datasheet.

    ab171820 has not yet been referenced specifically in any publications.

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