Anti-eIF4B antibody (ab171820)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-eIF4B antibody
See all eIF4B primary antibodies -
DescriptionMouse polyclonal to eIF4B
-
Host speciesMouse
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse
-
Immunogen
Full length protein corresponding to Human eIF4B aa 1-611.
Sequence:MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGD VSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFL GNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSL LSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWR ARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPCRDMD RYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYED RYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSAS TSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRP RERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENET LNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDT EQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDG GNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDG EDENEGEDYAE
Database link: AAH98437.1 -
Positive control
- eIF4B-transfected 293T cell lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.2
Constituent: 100% PBS -
Concentration information loading... -
PurityProtein A purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab171820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. |
Target
-
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
-
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
-
Post-translational
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. - Information by UniProt
-
Database links
- Entrez Gene: 1975 Human
- Entrez Gene: 75705 Mouse
- Omim: 603928 Human
- SwissProt: P23588 Human
- SwissProt: Q8BGD9 Mouse
- Unigene: 648394 Human
- Unigene: 702041 Human
- Unigene: 290022 Mouse
see all -
Alternative names
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
see all
Images
-
Western blot - Anti-eIF4B antibody (ab171820)All lanes : Anti-eIF4B antibody (ab171820) at 1 µg/ml
Lane 1 : eIF4B-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Datasheets and documents
Protocols
References
ab171820 has not yet been referenced specifically in any publications.
