Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF4B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human eIF4B aa 352-611 (C terminal).
    Database link: P23588

  • Positive control

    • MCF7, 22RV-1, HeLa, 293T and M21 cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 69 kDa.
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310046H11Rik antibody
    • AL024095 antibody
    • C85189 antibody
    • EIF 4B antibody
    • eIF-4B antibody
    • EIF4B antibody
    • Eukaryotic initiation factor 4B antibody
    • Eukaryotic translation initiation factor 4B antibody
    • IF4B_HUMAN antibody
    • PRO1843 antibody
    see all

Images

  • All lanes : Anti-eIF4B antibody (ab186856) at 1/500 dilution

    Lane 1 : MCF7 cell extract
    Lane 2 : 22RV-1 cell extract
    Lane 3 : HeLa cell extract
    Lane 4 : 293T cell extract
    Lane 5 : M21 cell extract

    Predicted band size: 69 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling eIF4B with ab186856 at 1/200. Magnification: 400x.
  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186856. Blue DAPI for nuclear staining.

References

ab186856 has not yet been referenced specifically in any publications.

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