Product nameAnti-eIF4B antibody
See all eIF4B primary antibodies
DescriptionRabbit polyclonal to eIF4B
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human eIF4B aa 352-611 (C terminal).
Database link: P23588
- MCF7, 22RV-1, HeLa, 293T and M21 cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 69 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
- Information by UniProt
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
All lanes : Anti-eIF4B antibody (ab186856) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : 22RV-1 cell extract
Lane 3 : HeLa cell extract
Lane 4 : 293T cell extract
Lane 5 : M21 cell extract
Predicted band size: 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling eIF4B with ab186856 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186856. Blue DAPI for nuclear staining.
ab186856 has not yet been referenced specifically in any publications.