Key features and details
- Rabbit polyclonal to eIF4B
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4B antibody
See all eIF4B primary antibodies
DescriptionRabbit polyclonal to eIF4B
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human eIF4B aa 561-611. The exact sequence is proprietary. NP_001408.2
Database link: P23588
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245618 was affinity purified using an epitope specific to eIF4B immobilized on solid support.
Our Abpromise guarantee covers the use of ab245618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
- Information by UniProt
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All lanes : Anti-eIF4B antibody (ab245618) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Exposure time: 10 seconds
eIF4B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245618 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245618 at 1 μg/ml.
Lane 1: ab245618 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245618 has not yet been referenced specifically in any publications.