Product nameAnti-eIF4B antibody [EP2299Y]
See all eIF4B primary antibodies
DescriptionRabbit monoclonal [EP2299Y] to eIF4B
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human eIF4B (C terminal). The exact sequence is proprietary.
- HeLa cell lysate, human prostate adenocarcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/200000. Detects a band of approximately 90 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
- Information by UniProt
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
Anti-eIF4B antibody [EP2299Y] (ab68474) at 1/200000 dilution + HeLa cell lysate at 10 µg
HRP conjugated Goat anti-rabbit. at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Human prostate adenocarcinoma stained with ab68474 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Albornoz A et al. The stress granule component TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation. J Virol 88:6611-22 (2014). ICC/IF ; Human . Read more (PubMed: 24696465) »