Anti-eIF4B antibody [EP2299Y] (ab68474)
![Western blot - Anti-eIF4B antibody [EP2299Y] (ab68474)](https://www.abcam.com/ps/products/68/ab68474/Images/ab68474-82768-anti-eif4b-antibody-ep2299y-western-blot.jpg "Western blot - Anti-eIF4B antibody [EP2299Y] (ab68474)")
Key features and details
- Rabbit monoclonal [EP2299Y] to eIF4B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4B antibody [EP2299Y]
See all eIF4B primary antibodies -
Description
Rabbit monoclonal [EP2299Y] to eIF4B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human eIF4B (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa cell lysate, human prostate adenocarcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2299Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Buffer
Applications
Our Abpromise guarantee covers the use of ab68474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200000. Detects a band of approximately 90 kDa (predicted molecular weight: 69 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. - Information by UniProt
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Database links
- Entrez Gene: 1975 Human
- Omim: 603928 Human
- SwissProt: P23588 Human
- Unigene: 648394 Human
- Unigene: 702041 Human
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Alternative names
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
see all
Images
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Western blot - Anti-eIF4B antibody [EP2299Y] (ab68474)Anti-eIF4B antibody [EP2299Y] (ab68474) at 1/200000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP conjugated Goat anti-rabbit. at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] (ab68474)](https://www.abcam.com/ps/products/68/ab68474/Images/ab68474-82767-anti-eif4b-antibody-ep2299y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] (ab68474)Human prostate adenocarcinoma stained with ab68474 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (1)
ab68474 has been referenced in 1 publication.
- Albornoz A et al. The stress granule component TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation. J Virol 88:6611-22 (2014). ICC/IF ; Human . PubMed: 24696465
