Overview

  • Product name

    Anti-eIF4B (phospho S406) antibody [EPR7683]
    See all eIF4B primary antibodies
  • Description

    Rabbit monoclonal [EPR7683] to eIF4B (phospho S406)
  • Host species

    Rabbit
  • Specificity

    ab134138 only detects eIF4B phosphorylated at Serine 406.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Specific peptide corresponding to residues surrounding phosphorylated Serine 406 of Human eIF4B.(Uniprot ID: P23588)

  • Positive control

    • HeLa cell lysate and Human colon tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.35 x 10 -10 M
    Learn more about KD
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7683
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
    • Sequence similarities

      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications

      Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2310046H11Rik antibody
      • AL024095 antibody
      • C85189 antibody
      • EIF 4B antibody
      • eIF-4B antibody
      • EIF4B antibody
      • Eukaryotic initiation factor 4B antibody
      • Eukaryotic translation initiation factor 4B antibody
      • IF4B_HUMAN antibody
      • PRO1843 antibody
      see all

    Images

    • All lanes : Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138) at 1/10000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Ani-rabbit HRP at 1/2000 dilution

      Predicted band size: 69 kDa

    • Immunohistochemical analysis of Paraffin-embedded Human colon tissue labelling eIF4B phosphorylated at S406 with ab134138 at 1/50 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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