Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7683] to eIF4B (phospho S406)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-eIF4B (phospho S406) antibody [EPR7683]
See all eIF4B primary antibodies
DescriptionRabbit monoclonal [EPR7683] to eIF4B (phospho S406)
Specificityab134138 only detects eIF4B phosphorylated at Serine 406.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Specific peptide corresponding to residues surrounding phosphorylated Serine 406 of Human eIF4B.(Uniprot ID: P23588)
- HeLa cell lysate and Human colon tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.35 x 10 -10 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
- Information by UniProt
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All lanes : Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138) at 1/10000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : Goat Ani-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Immunohistochemical analysis of Paraffin-embedded Human colon tissue labelling eIF4B phosphorylated at S406 with ab134138 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134138 has been referenced in 1 publication.
- Lee JS et al. Loss of SLC25A11 causes suppression of NSCLC and melanoma tumor formation. EBioMedicine 40:184-197 (2019). PubMed: 30686754