Product nameAnti-eIF4B (phospho S504) antibody [EP2261Y]
See all eIF4B primary antibodies
DescriptionRabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
Specificityab75823 detects eIF4B phosphorylated at serine 504.
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B
- 293T cell lysates, untreated or treated with AP.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
|ICC||1/100 - 1/250.|
FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
- Information by UniProt
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with AP
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa