Anti-eIF4E antibody [5D11] (ab171091)
Key features and details
- Mouse monoclonal [5D11] to eIF4E
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-eIF4E antibody [5D11]
See all eIF4E primary antibodies -
Description
Mouse monoclonal [5D11] to eIF4E -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant full length protein corresponding to Human eIF4E. Produced in HEK293T cells.
Database link: P06730 -
Positive control
- Hela, MCF7, K562, Jurkat, U2OS and HepG2 lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.1% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5D11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500.
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WB | (5) |
1/1000. Predicted molecular weight: 25 kDa.
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IP |
Use at 6 µg/mg of lysate.
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Notes |
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ICC/IF
1/100 - 1/500. |
WB
1/1000. Predicted molecular weight: 25 kDa. |
IP
Use at 6 µg/mg of lysate. |
Target
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Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family. -
Post-translational
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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Database links
- Entrez Gene: 1977 Human
- Entrez Gene: 13684 Mouse
- Omim: 133440 Human
- SwissProt: P06730 Human
- SwissProt: P63073 Mouse
- Unigene: 249718 Human
- Unigene: 3941 Mouse
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Alternative names
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
Images
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All lanes : Anti-eIF4E antibody [5D11] (ab171091) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : U2OS whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lane 8 : NIH3T3 whole cell lysate
Lane 9 : NRK whole cell lysate
Lysates/proteins at 80 µg per lane.
Secondary
All lanes : Goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa -
ab171091 staining eIF4E (green) in the cytoplasm of MCF-7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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ab171091 staining eIF4E (green) in the cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab171091 has been referenced in 4 publications.
- Yang S et al. LINC00035 Transcriptional Regulation of SLC16A3 via CEBPB Affects Glycolysis and Cell Apoptosis in Ovarian Cancer. Evid Based Complement Alternat Med 2021:5802082 (2021). PubMed: 34671407
- Qi NN et al. Up-regulation of microRNA-496 suppresses proliferation, invasion, migration and in vivo tumorigenicity of human osteosarcoma cells by targeting eIF4E. Biochimie 163:1-11 (2019). PubMed: 30998968
- Jiang L et al. Long non-coding RNA RP11-468E2.5 curtails colorectal cancer cell proliferation and stimulates apoptosis via the JAK/STAT signaling pathway by targeting STAT5 and STAT6. J Exp Clin Cancer Res 38:465 (2019). PubMed: 31718693
- Choi JH et al. hnRNP Q regulates IRES-mediated fmr1 translation in neurons. Mol Cell Biol N/A:N/A (2018). PubMed: 30478144