Product nameAnti-eIF4E antibody [5D11]
See all eIF4E primary antibodies
DescriptionMouse monoclonal [5D11] to eIF4E
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Does not react with: Rat
Recombinant full length protein corresponding to Human eIF4E. Produced in HEK293T cells.
Database link: P06730
- Hela, MCF7, K562, Jurkat, U2OS and HepG2 lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 30% Glycerol, 0.1% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab171091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/1000. Predicted molecular weight: 25 kDa.|
|IP||Use at 6 µg/mg of lysate.|
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Information by UniProt
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
All lanes : Anti-eIF4E antibody [5D11] (ab171091) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : U2OS whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lane 8 : NIH3T3 whole cell lysate
Lane 9 : NRK whole cell lysate
Lysates/proteins at 80 µg per lane.
All lanes : Goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
ab171091 staining eIF4E (green) in the cytoplasm of MCF-7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
ab171091 staining eIF4E (green) in the cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
This product has been referenced in:
- Qi NN et al. Up-regulation of microRNA-496 suppresses proliferation, invasion, migration and in vivo tumorigenicity of human osteosarcoma cells by targeting eIF4E. Biochimie 163:1-11 (2019). Read more (PubMed: 30998968) »
- Choi JH et al. hnRNP Q regulates IRES-mediated fmr1 translation in neurons. Mol Cell Biol N/A:N/A (2018). Read more (PubMed: 30478144) »