- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-eIF4E antibody
See all eIF4E primary antibodies -
DescriptionRabbit polyclonal to eIF4E
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-P, ELISA, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein (Human).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/1000. | |
| WB | 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | 1/1000. | |
| ICC/IF | Use at an assay dependent concentration. See Abreview. |
Target
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FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
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Post-translational
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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Database links
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: P06730 Human
- Unigene: 249718 Human
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Alternative names
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
Images
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Western blot - Anti-eIF4E antibody (ab1126)Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract
Predicted band size: 25 kDa
Observed band size: 28 kDa (why is the actual band size different from the predicted?)
Western blot of 30µ g bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa). -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody (ab1126)This image is courtesy of an anonymous Abreviewab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
This product has been referenced in:
- Zhang P et al. p53, MDM2, eIF4E and EGFR expression in nasopharyngeal carcinoma and their correlation with clinicopathological characteristics and prognosis: A retrospective study. Oncol Lett 9:113-118 (2015). IHC ; Human . Read more (PubMed: 25435943) »
- Chen B et al. Knockdown of eukaryotic translation initiation factor 4E suppresses cell growth and invasion, and induces apoptosis and cell cycle arrest in a human lung adenocarcinoma cell line. Mol Med Rep 12:7971-8 (2015). WB . Read more (PubMed: 26498338) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"