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    eif4e-antibody-ab1126.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-eIF4E antibody (ab1126)

  • Datasheet
Reviews (4)Q&A (6)References (13)

Product price, shipping and contact information

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Find out more.

Western blot - Anti-eIF4E antibody (ab1126)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)

Key features and details

  • Rabbit polyclonal to eIF4E
  • Suitable for: WB, IHC-P
  • Reacts with: Cow, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-eIF4E antibody
    See all eIF4E primary antibodies
  • Description

    Rabbit polyclonal to eIF4E
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Cow
    See all applications and species data
  • Immunogen

    Recombinant full length protein (Human).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human eIF4E protein (ab56276)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Cow
Application Abreviews Notes
WB (2)
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities

    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications

    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Target information above from: UniProt accession P06730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281751 Cow
    • Entrez Gene: 1977 Human
    • Omim: 133440 Human
    • SwissProt: Q9N0T5 Cow
    • SwissProt: P06730 Human
    • Unigene: 249718 Human
    • Alternative names

      • AUTS19 antibody
      • CBP antibody
      • eIF 4E antibody
      • eIF 4F 25 kDa subunit antibody
      • EIF 4F antibody
      • eIF-4E antibody
      • eIF-4F 25 kDa subunit antibody
      • eIF4E antibody
      • EIF4E1 antibody
      • EIF4EL1 antibody
      • EIF4F antibody
      • Eukaryotic translation initiation factor 4 E antibody
      • Eukaryotic translation initiation factor 4E antibody
      • Eukaryotic translation initiation factor 4E like 1 antibody
      • IF4E_HUMAN antibody
      • Messanger RNA Cap Binding Protein eIF 4E antibody
      • MGC111573 antibody
      • mRNA cap binding protein antibody
      • mRNA cap-binding protein antibody
      see all

    Images

    • Western blot - Anti-eIF4E antibody (ab1126)
      Western blot - Anti-eIF4E antibody (ab1126)
      Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract

      Predicted band size: 25 kDa
      Observed band size: 28 kDa
      why is the actual band size different from the predicted?



       Western blot of 30 µg bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)
      IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (13)

    Publishing research using ab1126? Please let us know so that we can cite the reference in this datasheet.

    ab1126 has been referenced in 13 publications.

    • Batool A  et al. Eukaryotic Initiation Factor 4E phosphorylation acts a switch for its binding to 4E-BP1 and mRNA cap assembly. Biochem Biophys Res Commun 527:489-495 (2020). PubMed: 32336547
    • Pitchiaya S  et al. Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality. Mol Cell 74:521-533.e6 (2019). PubMed: 30952514
    • Sun Y  et al. Newcastle disease virus induces stable formation of bona fide stress granules to facilitate viral replication through manipulating host protein translation. FASEB J 31:1337-1353 (2017). PubMed: 28011649
    • Schunter AJ  et al. Phosphoproteomics of colon cancer metastasis: comparative mass spectrometric analysis of the isogenic primary and metastatic cell lines SW480 and SW620. Anal Bioanal Chem 409:1749-1763 (2017). PubMed: 27987026
    • Gujrati M  et al. Targeted Dual pH-Sensitive Lipid ECO/siRNA Self-Assembly Nanoparticles Facilitate In Vivo Cytosolic sieIF4E Delivery and Overcome Paclitaxel Resistance in Breast Cancer Therapy. Adv Healthc Mater 5:2882-2895 (2016). PubMed: 27723260
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Question

    Thank you for your email. The issue for AB1126 is for the same customer. This started from August 2011.

    This customer did receive a replacement of 1 vial form the same Lot# but initially he ordered 2, and this also failed for him.

    I hope to hear from you soon.



    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 27 2012

    Answer

    Thank you for your response.
    This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of two vials of ab1126. For your information, the internal reference note for this credit is CN19459. You can use this credit in the near future for any of the products which are in the catalogue.
    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jan 27 2012

    Question

    Dear Technical team Good day! We have a customer who has purchased the above antibody and would like to know the following information: As your website recommended the tissue and dilution of antibody I have done work like that. Process: Antibody dil- 1/1000 Breast Cancer Tissue Mild and Standard Retrieval time Automated Benchmark machine for imunostaining Result: With standard Retrieval time- strong peri nuclear staining but non specific with cytoplasmic as well. With Mild retrieval time- Not specific staining.   Please check with my questions: 1. What kind of breast cancer tissue is suitable for this antibody as control? 2. Which retrieval time is suitable? 3. Which antibody dilution is suitable? 4. Is it tested on other cancer tissue? 5. What is the actual peri nuclear staining?   I hope to hear from you soon. Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2011

    Answer

    Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. As it can be seen from the image in the datasheet, human breast carcinoma is a good option for a positive control. However, there are other cancer tissues that may also give successful results as positive control, these are: colorectal cancer tissue and prostate cancer tissue, you can find the information at the Human Atlas link: http://www.proteinatlas.org/ENSG00000151247 Regarding the retrieval time, it really has to be optimised for each case. The standard recommended time is 20 minutes, less than 20 minutes may leave the antigens under retrieved, leading to weak staining. More than 20 minutes may leave them over-retrieved, leading to nonspecific background staining and also increasing the chances of sections dissociating from the slides. A control experiment is recommended beforehand, where slides of the same tissue section are retrieved for 5, 10, 15, 20, 25 and 30 minutes before being immunohistochemically stained to evaluate optimum antigen retrieval time for the particular antibody being used. In the following link you will find useful information for the different antigen retrieval methods: https://www.abcam.com/index.html?pageconfig=resource&rid=11488#notes2 The optimal dilution for the primary antibody needs also to be optimised for every experiment. We suggest using 1/1000 as a starting point, and depending on the results you may need to use different dilutions. All the information about this product is on the datasheet, we will be glad to update it with new data from customers. All new images and publications are welcome in order to provide more information to researches. I would be very happy to help you with your experiments, but in order to do it I will appreciate to gather more information. I send you a questionnaire that only takes about 5 minutes to be filled. Any images will also be very helpful to understand why this antibody did not perform as stated in the datasheet. All of our products are covered by our Abpromise guarantee. In case they fail to perform as expected, we will offer a free of charge replacement, a reimbursement or a credit note. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2011

    Western blot abreview for Anti-eIF4E antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLA)
    Loading amount
    20 µg
    Specification
    HeLA
    Gel Running Conditions
    Reduced Denaturing (15% Tris-tricine gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 06 2011

    Question

    Dear Technical,   Good day! I was wondering if you could give us a recommended dilution for IHC-P for this antibody. As the recommended dilution states to use 1ug/ml but it is a bit difficult when the concentration of the antibody is not recommended. Could you please help me recommend a dilution for a customer?   I hope to hear from you soon.   Kind regards  

    Read More

    Abcam community

    Verified customer

    Asked on Aug 22 2011

    Answer

    Thank you for your enquiry regarding ab1126. I can confirm that the antibody has been tested in IHC-P at a dilution of 1/1000 (instead of 1ug/ml). The datasheet (application notes) and the legend of the IHC-P image has been amended accordingly. I apologize for any inconvenience caused and thank you for bringing this matter to our attention.

    Read More

    Abcam Scientific Support

    Answered on Aug 22 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4E antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Epithelial)
    Specification
    Epithelial
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton-X 100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 05 2007

    Question

    I have purchased a number of antibodies from Abcam and they have all been good. I have couple of questions which i would like you to assist me with, as i have checked your website and found no answers. The Rabbit polyclonal eIF4E antibody (ab1126) does it detect total eIF4E ie the non phospho and the phospho or just the non phospho form The Mouse Monoclonal eIF2a antibody (ab5369) does it detect total eIF2a ie the non phospho and the phospo or just the non phospho form

    Read More

    Abcam community

    Verified customer

    Asked on Feb 05 2007

    Answer

    Thank you for your enquiry. I can confirm that both these eIF2a antibodies (ab1126, ab5369)will detect both phosphorylated and de-phosphorylated forms of the protein. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Feb 09 2007

    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4E antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Blocking step
    1% BSA + 1.5% normal goat serum as blocking agent for 30 minute(s) · Concentration: 1
    Read More

    Dr. Karppiah Muthumani

    Verified customer

    Submitted Nov 17 2006

    Western blot abreview for Anti-eIF4E antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HeLa)
    Loading amount
    50 µg
    Specification
    HeLa
    Treatment
    rVpr & LMB
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
    Read More

    Dr. Karppiah Muthumani

    Verified customer

    Submitted Nov 17 2006

    Question

    Thanks for your rapid reply, it was a misunderstandig. Of course we know, that eIF4E is well expressed in HeLa. and we used for eIF4E a little aliquot of an ab which we got as a present from another lab. So previously we could detect eIF4E. The point is your ab results in a completly black western, even when I expose the film only for 5 sec. unfortunately we don't have any bovine kidney extract to test that your ab. regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2002

    Answer

    Thank you very much for your e-mail. I would like to reassure you that if an antibody does not perform as specified on the datasheet, we will provide a full refund or offer you a replacement of equivalent value. I have contacted the supplier regarding your problem and will get back to you as soon as they reply. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

    Read More

    Abcam Scientific Support

    Answered on Oct 08 2002

    Question

    I have used the anti-eIF4E FROM NEB-CST, which although recommended for immunoprecipitation does not work for this application in my hands. Is your antibody definitely capable of immunoprecipitating from cell lysates and not just purified protein? Is it made to full length recombinant protein? many thanks

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2002

    Answer

    Yes, our antibody is capable of precipitating eIF-4E from cell lysates. The antibody was made to the full length recombinant human protein.

    Read More

    Abcam Scientific Support

    Answered on Sep 13 2002

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