Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF4E
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab1126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/1000.
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
ICC/IF Use at an assay dependent concentration. See Abreview.

Target

  • Function
    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities
    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Images

  • Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract

    Predicted band size: 25 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



     Western blot of 30 µg bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa).
  • ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.

    See Abreview

  • IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Schunter AJ  et al. Phosphoproteomics of colon cancer metastasis: comparative mass spectrometric analysis of the isogenic primary and metastatic cell lines SW480 and SW620. Anal Bioanal Chem 409:1749-1763 (2017). Read more (PubMed: 27987026) »
  • Gujrati M  et al. Targeted Dual pH-Sensitive Lipid ECO/siRNA Self-Assembly Nanoparticles Facilitate In Vivo Cytosolic sieIF4E Delivery and Overcome Paclitaxel Resistance in Breast Cancer Therapy. Adv Healthc Mater 5:2882-2895 (2016). Read more (PubMed: 27723260) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

Thank you for your response.
This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of two vials of ab1126. For your information, the internal reference note for this credit is CN19459. You can use this credit in the near future for any of the products which are in the catalogue.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. As it can be seen from the image in the datasheet, human breast carcinoma is a good option for a positive control. However, there are other cancer tissues that may also give successful results as positive control, these are: colorectal cancer tissue and prostate cancer tissue, you can find the information at the Human Atlas link: http://www.proteinatlas.org/ENSG00000151247 Regarding the retrieval time, it really has to be optimised for each case. The standard recommended time is 20 minutes, less than 20 minutes may leave the antigens under retrieved, leading to weak staining. More than 20 minutes may leave them over-retrieved, leading to nonspecific background staining and also increasing the chances of sections dissociating from the slides. A control experiment is recommended beforehand, where slides of the same tissue section are retrieved for 5, 10, 15, 20, 25 and 30 minutes before being immunohistochemically stained to evaluate optimum antigen retrieval time for the particular antibody being used. In the following link you will find useful information for the different antigen retrieval methods: https://www.abcam.com/index.html?pageconfig=resource&rid=11488#notes2 The optimal dilution for the primary antibody needs also to be optimised for every experiment. We suggest using 1/1000 as a starting point, and depending on the results you may need to use different dilutions. All the information about this product is on the datasheet, we will be glad to update it with new data from customers. All new images and publications are welcome in order to provide more information to researches. I would be very happy to help you with your experiments, but in order to do it I will appreciate to gather more information. I send you a questionnaire that only takes about 5 minutes to be filled. Any images will also be very helpful to understand why this antibody did not perform as stated in the datasheet. All of our products are covered by our Abpromise guarantee. In case they fail to perform as expected, we will offer a free of charge replacement, a reimbursement or a credit note. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLA)
Loading amount
20 µg
Specification
HeLA
Gel Running Conditions
Reduced Denaturing (15% Tris-tricine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 06 2011

Answer

Thank you for your enquiry regarding ab1126. I can confirm that the antibody has been tested in IHC-P at a dilution of 1/1000 (instead of 1ug/ml). The datasheet (application notes) and the legend of the IHC-P image has been amended accordingly. I apologize for any inconvenience caused and thank you for bringing this matter to our attention.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Epithelial)
Specification
Epithelial
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 05 2007

Answer

Thank you for your enquiry. I can confirm that both these eIF2a antibodies (ab1126, ab5369)will detect both phosphorylated and de-phosphorylated forms of the protein. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Blocking step
1% BSA + 1.5% normal goat serum as blocking agent for 30 minute(s) · Concentration: 1

Dr. Karppiah Muthumani

Verified customer

Submitted Nov 17 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Loading amount
50 µg
Specification
HeLa
Treatment
rVpr & LMB
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5

Dr. Karppiah Muthumani

Verified customer

Submitted Nov 17 2006

Answer

Thank you very much for your e-mail. I would like to reassure you that if an antibody does not perform as specified on the datasheet, we will provide a full refund or offer you a replacement of equivalent value. I have contacted the supplier regarding your problem and will get back to you as soon as they reply. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

Read More

Answer

Yes, our antibody is capable of precipitating eIF-4E from cell lysates. The antibody was made to the full length recombinant human protein.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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