Key features and details
- Chicken polyclonal to eIF4E
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgY
Product nameAnti-eIF4E antibody
See all eIF4E primary antibodies
DescriptionChicken polyclonal to eIF4E
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide. This information is considered to be commercially sensitive.
- WG: MOLT4, Daudi and HeLa whole cell lysate. IHC-P: Human lung adenocarcinoma tissue. Human cerebellum tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.03% Sodium phosphate, 0.87% Sodium chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab235191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Information by UniProt
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for eIF4E using ab235191 at 1/1000 in immunohistochemistry. Counterstained with hematoxylin. HIER performed.
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for eIF4E using ab235191 at 1/100 for 30mins at RT in immunohistochemistry. Counterstained with hematoxylin, DAB staining. HIER performed using citrate buffer 20mins.
All lanes : Anti-eIF4E antibody (ab235191) at 5 µg/ml
Lane 1 : MOLT4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Chicken IgG HRP at 1/40000 dilution
Predicted band size: 25 kDa
Exposure time: 60 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235191 has not yet been referenced specifically in any publications.