Overview

  • Product name

  • Description

    Chicken polyclonal to eIF4E
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WG: MOLT4, Daudi and HeLa whole cell lysate. IHC-P: Human lung adenocarcinoma tissue. Human cerebellum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities

    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications

    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for eIF4E using ab235191 at 1/1000 in immunohistochemistry. Counterstained with hematoxylin. HIER performed.

  • Formalin-fixed, paraffin-embedded human cerebellum tissue stained for eIF4E using ab235191 at 1/100 for 30mins at RT in immunohistochemistry. Counterstained with hematoxylin, DAB staining. HIER performed using citrate buffer 20mins.

  • All lanes : Anti-eIF4E antibody (ab235191) at 5 µg/ml

    Lane 1 : MOLT4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-Chicken IgG HRP at 1/40000 dilution

    Predicted band size: 25 kDa


    Exposure time: 60 seconds

References

ab235191 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235191.
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