Product nameAnti-eIF4E antibody
See all eIF4E primary antibodies
DescriptionRabbit polyclonal to eIF4E
Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: HL-60, SW620, MCF7 and HEK-293T whole cell lysate; Mouse brain lysate; Rat testis lysate. IHC-P: Human lung cancer and stomach tissue. ICC/IF: U-2 OS cells. IP: HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab238519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 25 kDa.|
|IP||1/50 - 1/100.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/100.|
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Information by UniProt
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
All lanes : Anti-eIF4E antibody (ab238519) at 1/1000 dilution
Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 2 : SW620 whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Rat testis tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 10 seconds
Blocking buffer: 3% non-fat dry milk in TBST.
U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for eIF4E (Green) using ab238519 at 1/100 dilution in ICC/IF analysis. Nuclear counterstain was DAPI (Blue).
eIF4E was immunoprecipitated from 200 μg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell extract using 3 μg of ab238519. Western blot was performed from the immunoprecipitate using ab238519 at 1/1000 dilution.
Lane 1: HEK-293T cell extract (input).
Lane 2: Control IgG instead of ab238519 in HEK-293T cell extract.
Lane 3: ab238519 IP in HEK-293T cell extract.
Paraffin-embedded human stomach tissue stained for eIF4E using ab238519 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung cancer tissue stained for eIF4E using ab238519 at 1/100 dilution in immunohistochemical analysis.
ab238519 has not yet been referenced specifically in any publications.