Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4E antibody
See all eIF4E primary antibodies
DescriptionRabbit polyclonal to eIF4E
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Elephant
Synthetic peptide corresponding to a region between residue 1 and 50 of human eIF4E (NP_001959.1).
- HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72116 was affinity purified using an epitope specific to eIF4E immobilized on solid support.
Our Abpromise guarantee covers the use of ab72116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Information by UniProt
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
ab72116 has not yet been referenced specifically in any publications.