• Product name
    Anti-eIF4E antibody [Y448]
    See all eIF4E primary antibodies
  • Description
    Rabbit monoclonal [Y448] to eIF4E
  • Host species
  • Specificity
    The antibody detects a band on western blot of approximately 28 kDa.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF4E aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • 293 cell lysate, human breast carcinoma.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab33766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities
    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all


  • All lanes : Anti-eIF4E antibody [Y448] (ab33766) at 0.025 µg/ml

    Lane 1 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysate
    Lane 2 : C6 (Rat glioma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa

    Blocking and diluting buffer: 5% NFDM/TBST. 

    Exposure time - Lane 1: 160 seconds
                             Lane 2: 70 seconds 

  • Anti-eIF4E antibody [Y448] (ab33766) at 1/500 dilution + 293 cell lysate

    Predicted band size: 25 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • This image shows human breast carcinoma stained with ab33766
  • Overlay histogram showing HEK293 cells stained with ab33766 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33766, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

  • ICC/IF image of ab33766 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33766, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Li F  et al. Expression of 4E-BP1 and phospho-4E-BP1 correlates with the prognosis of patients with clear cell renal carcinoma. Cancer Manag Res 10:1553-1563 (2018). Read more (PubMed: 29942157) »
  • Tamarkin-Ben-Harush A  et al. Cap-proximal nucleotides via differential eIF4E binding and alternative promoter usage mediate translational response to energy stress. Elife 6:N/A (2017). Read more (PubMed: 28177284) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Total protein in input
600000 cells
Immuno-precipitation step
Other - Dynabeads
mouse embryonic stem cells (J1)

Abcam user community

Verified customer

Submitted Aug 03 2018

Western blot
Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
mouse embryonic stem cells (J1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 10 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Mouse Cell (T cells)
T cells
Gating Strategy
Yes - Phosflow PermBuffer III

Dr. Beata Zygmunt

Verified customer

Submitted Jun 25 2013


Thank you for your enquiry. In most cases, I would recommend using milk, because it works just as good as BSA and it is a bit cheaper. In the case of eIF4e though, because it is a phosphoprotein, you might get unspecific binding using milk because milk contains a phospoprotein called casein. This would lead to very high background, so in this case I would recommend BSA to block. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. After contacting the originator of the antibody, I have updated the data sheet with the amino acid sequence which is NQEVANPEHYIKHPLQNR, which is amino acid residues 25-42, and it is specific for the N-terminus. I hope that this information helps. Please don't hesitate to ask us for help in the future.

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