Western blot abreview for Anti-eIF4E antibody [Y449]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells (J1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/2500
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 35 kDa

Additional data

Additional Notes
12% SDS-PAGE Gel, transfer with 85V for 1 hour.

Abcam user community

Verified customer

Submitted Apr 10 2018

Sign up