Overview

  • Product name

    Anti-eIF4E (phospho S209) antibody
    See all eIF4E primary antibodies
  • Description

    Rabbit polyclonal to eIF4E (phospho S209)
  • Host species

    Rabbit
  • Specificity

    ab131513 detects endogenous levels of eIF4E only when phosphorylated at Serine 209.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region around the phosphorylation site of Serine 209 (S-G-Sp-T-T) of Human eIF4E (NP_001124150.1).

  • Positive control

    • Methanol fixed MCF cells; HT29 cell extracts treated with Anisomycin; Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities

    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications

    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Images

  • All lanes : Anti-eIF4E (phospho S209) antibody (ab131513) at 1/500 dilution

    Lane 1 : HT29 cell extracts, untreated
    Lane 2 : HT29 cell extracts Anisomycin treated

    Predicted band size: 25 kDa



    For western blotting, membrane should be incubated with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight.
  • Immunofluorescence analysis of eIF4E (phospho S209) in methanol fixed MCF cells stained with ab131513 at a dilution of 1/100.
  • Immunohistochemical analysis of eIF4E (phospho S209) in paraffin embedded Human breast carcinoma tissue stained with ab131513 at a 1/50 dilution (left image), or preincubated with immunizing peptide (right image).

References

ab131513 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131513.
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