Key features and details
- Rabbit polyclonal to eIF4E (phospho S209)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4E (phospho S209) antibody
See all eIF4E primary antibodies
DescriptionRabbit polyclonal to eIF4E (phospho S209)
Specificityab131513 detects endogenous levels of eIF4E only when phosphorylated at Serine 209.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Methanol fixed MCF cells; HT29 cell extracts treated with Anisomycin; Human breast carcinoma tissue
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131513 was purified using epitope specific phosphopeptide. Non phospho specific antibodies were removed by chromatography using non phosphopeptide.
Our Abpromise guarantee covers the use of ab131513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Information by UniProt
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
All lanes : Anti-eIF4E (phospho S209) antibody (ab131513) at 1/500 dilution
Lane 1 : HT29 cell extracts, untreated
Lane 2 : HT29 cell extracts Anisomycin treated
Predicted band size: 25 kDa
For western blotting, membrane should be incubated with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight.
Immunofluorescence analysis of eIF4E (phospho S209) in methanol fixed MCF cells stained with ab131513 at a dilution of 1/100.
Immunohistochemical analysis of eIF4E (phospho S209) in paraffin embedded Human breast carcinoma tissue stained with ab131513 at a 1/50 dilution (left image), or preincubated with immunizing peptide (right image).
ab131513 has not yet been referenced specifically in any publications.