Recombinant Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2169Y] to eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]
See all eIF4EBP1 + eIF4EBP2 + eIF4EBP3 primary antibodies -
Description
Rabbit monoclonal [EPR2169Y] to eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T cell lysate IHC-P: Human breast carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2169Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/10000. Detects a band of approximately 14-19 kDa (predicted molecular weight: 13 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/5000 - 1/10000. Detects a band of approximately 14-19 kDa (predicted molecular weight: 13 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
eIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. eIF4EBP2 regulates eIF4E activity by preventing its assembly into the EIF4F complex. It mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. Nonphosphorylated EIF4EBP2 interacts with EIF4E. eIF4EBP2 is phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF, and belongs to the eIF4E binding protein family. eIF4EBP3 is a member of the EIF4EBP family which derives it name from proteins that bind to eukaryotic initiation factor 4E and that prevent its assembly into EIF4F. Cotranscription of this gene and the neighboring upstream gene (MASK) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C terminus due to an alternate reading frame for the EIF4EBP3 segments. -
Database links
- Entrez Gene: 1978 Human
- Entrez Gene: 1979 Human
- Entrez Gene: 8637 Human
- SwissProt: O60516 Human
- SwissProt: Q13541 Human
- SwissProt: Q13542 Human
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Alternative names
- 4EBP1 antibody
- 4EBP2 antibody
- 4EBP3 antibody
see all
Images
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All lanes : Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with FBS
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
Observed band size: 14-19 kDa why is the actual band size different from the predicted? -
Human breast carcinoma tissue stained with ab68187 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab68187 has been referenced in 6 publications.
- Li Y et al. Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E. Development 148:N/A (2021). PubMed: 34013332
- Freis P et al. mTOR inhibitors activate PERK signaling and favor viability of gastrointestinal neuroendocrine cell lines. Oncotarget 8:20974-20987 (2017). WB . PubMed: 28423496
- Zhang X et al. Targeting CD47 and Autophagy Elicited Enhanced Antitumor Effects in Non-Small Cell Lung Cancer. Cancer Immunol Res 5:363-375 (2017). PubMed: 28351890
- Lin TV et al. Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Proc Natl Acad Sci U S A 113:11330-11335 (2016). IHC-FrFl ; Mouse . PubMed: 27647922
- Wang S et al. The role of autophagy in the neurotoxicity of cationic PAMAM dendrimers. Biomaterials 35:7588-97 (2014). PubMed: 24906346
- Fan J et al. Autophagy Plays a Critical Role in ChLym-1-Induced Cytotoxicity of Non-Hodgkin's Lymphoma Cells. PLoS One 8:e72478 (2013). WB ; Human . PubMed: 24015249