Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF4EBP1
  • Host species

    Rabbit
  • Specificity

    ab131453 detects endogenous levels of eIF4EBP1 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide counjugated to KLH, surrounding amino acids 43-47 (S-T-T-P-G) of Human eIF4EBP1 (NP_004086.1).

  • Positive control

    • Methanol fixed HeLa cells; 293 and HepG2 cell extracts; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • Sequence similarities

    Belongs to the eIF4E-binding protein family.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Images

  • All lanes : Anti-eIF4EBP1 antibody (ab131453) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : HepG2 cell extracts

    Predicted band size: 13 kDa

  • Immunofluorescent analysis of methanol fixed HeLa cells labelling eIF4EBP1 with ab131453 antibody at a dilution of 1/100.
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eIF4EBP1 with ab131453 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References

This product has been referenced in:

  • Cui C  et al. Znhit1 inhibits breast cancer by up-regulating PTEN to deactivate the PI3K/Akt/mTOR pathway. Life Sci 224:204-211 (2019). Read more (PubMed: 30928405) »
  • Chen C  et al. LncRNA HULC mediates radioresistance via autophagy in prostate cancer cells. Braz J Med Biol Res 51:e7080 (2018). Read more (PubMed: 29694502) »
See all 2 Publications for this product

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