Product nameAnti-eIF4EBP1 antibody [Y330]
See all eIF4EBP1 primary antibodies
DescriptionRabbit monoclonal [Y330] to eIF4EBP1
Specificityab32130 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS.
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IPmore details
Unsuitable for: ICC
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues near the C-term of human4E-BP1
- K562 cell lysate and human prostate carcinoma
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 15-20 kDa (predicted molecular weight: 12 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similaritiesBelongs to the eIF4E-binding protein family.
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: eIF4EBP1 knockout HAP1 cell lysate (20 µg)
Lane 3: Hek293 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32130 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32130 was shown to specifically react with eIF4EBP1 when eIF4EBP1 knockout samples were used. Wild-type and eIF4EBP1 knockout samples were subjected to SDS-PAGE. ab32130 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution + K562 cell lysate
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
ab32130, staining human prostate carcinoma by immunohistochemistry, Paraffin embedded tissue
This product has been referenced in:
- Lu Q et al. Akt inhibition attenuates rasfonin-induced autophagy and apoptosis through the glycolytic pathway in renal cancer cells. Cell Death Dis 6:e2005 (2015). Human . Read more (PubMed: 26633711) »
- Hu Y et al. MiR-99a antitumor activity in human breast cancer cells through targeting of mTOR expression. PLoS One 9:e92099 (2014). WB ; Human . Read more (PubMed: 24637915) »