Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-eIF4EBP1 antibody [Y330] - BSA and Azide free (ab173322)

Overview

  • Product name

    Anti-eIF4EBP1 antibody [Y330] - BSA and Azide free
    See all eIF4EBP1 primary antibodies
  • Description

    Rabbit monoclonal [Y330] to eIF4EBP1 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS. 

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, IPmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF4EBP1 aa 50-150 (C terminal). The exact sequence is proprietary.

  • General notes

    ab173322 is the carrier-free version of ab32130 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab173322 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 15-20 kDa (predicted molecular weight: 12 kDa).
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • Sequence similarities

      Belongs to the eIF4E-binding protein family.
    • Post-translational
      modifications

      Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all

    References

    ab173322 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173322.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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