Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T45)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4EBP1 (phospho T45) antibody
See all eIF4EBP1 primary antibodies
DescriptionRabbit polyclonal to eIF4EBP1 (phospho T45)
Specificityab47367 detects endogenous levels of 4EBP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4EBP2 and 4EBP3 when phosphorylated at equivalent sites.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
The antiserum was produced against synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
- MDA435 cells, breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab47367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 13 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similaritiesBelongs to the eIF4E-binding protein family.
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47367. Left hand image without immunising peptide; right hand image with immunising peptide.
Western blot analysis of extracts from MDA435 cells untreated (lane 2) or treated (lane 1) with EGF (200nm, 5min), using ab47367.
ab47367 has not yet been referenced specifically in any publications.