Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T46)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-eIF4EBP1 (phospho T46) antibody
See all eIF4EBP1 primary antibodies
DescriptionRabbit polyclonal to eIF4EBP1 (phospho T46)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human eIF4EBP1 (phospho T46).
- WB: PI3K inhibitor LY2904002 and PDGF treated and untreated NIH3T3 cell lysate. ICC/IF: 70% confluent log phase HeLa cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAb27792 was purified from rabbit serum by sequential epitope specific chromatography. The antibody was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated eIF4EBP1. The final product is generated by affinity chromatography using a eIF4EBP1 derived peptide that is phosphorylated at threonine 46.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 13 kDa.
1/1000. Predicted molecular weight: 13 kDa.
FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similaritiesBelongs to the eIF4E-binding protein family.
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two
hours at room temperature.)
Lane 1 : PDGF treated NIH3T3 cell lysate
Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate
All lanes : goat F(ab)2 anti rabbit IgG
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3: controls). Blocking step: 3% BSA TBST buffer for one hour at room temperature.
Immunofluorescence analysis of eIF4EBP1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with 4EBP1 (pT46) Rabbit Polyclonal Antibody (ab27792) at 1/250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
ab27792 has been referenced in 2 publications.
- Liu S et al. Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways. J Exp Clin Cancer Res 40:66 (2021). PubMed: 33579337
- Lauria F et al. SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Nat Cell Biol 22:1239-1251 (2020). PubMed: 32958857