Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T70)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4EBP1 (phospho T70) antibody
See all eIF4EBP1 primary antibodies
DescriptionRabbit polyclonal to eIF4EBP1 (phospho T70)
SpecificityDetects endogenous levels of eIF4EBP1 only when phosphorylated at threonine70.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide derived from human eIF4EBP1 (Q13541) around the phosphorylation site of Thr70 (T-K-TP-P-P).
- Human colon carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without calcium and magnesium
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
Our Abpromise guarantee covers the use of ab111421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similaritiesBelongs to the eIF4E-binding protein family.
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
ab111421 has not yet been referenced specifically in any publications.