Recombinant Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A]
See all eIF4EBP1 primary antibodies -
Description
Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysates treated with FBS.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR654(2)A -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa). |
Target
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Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. -
Sequence similarities
Belongs to the eIF4E-binding protein family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 1978 Human
- Omim: 602223 Human
- SwissProt: Q13541 Human
- Unigene: 411641 Human
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Alternative names
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
see all
Images
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All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831) at 1/500 dilution
Lane 1 : 293T cell lysates un-treated
Lane 2 : 293T cell lysates treated with FBS
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab75831 has been referenced in 4 publications.
- Morales-Garcia V et al. The high-risk HPV E6 proteins modify the activity of the eIF4E protein via the MEK/ERK and AKT/PKB pathways. FEBS Open Bio 10:2541-2552 (2020). PubMed: 32981220
- Jiang Y et al. TIPE2 governs macrophage polarization via negative regulation of mTORC1. Mol Med Rep 17:952-960 (2018). PubMed: 29115630
- Liu Y et al. Effects of bufalin on the mTOR/p70S6K pathway and apoptosis in esophageal squamous cell carcinoma in nude mice. Int J Mol Med 40:357-366 (2017). PubMed: 28656204
- Li S et al. Clinical and genetic analysis of tuberous sclerosis complex-associated renal angiomyolipoma in Chinese pedigrees. Oncol Lett 14:7085-7090 (2017). PubMed: 29344138