Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)


  • Product name
    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A]
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70)
  • Host species
  • Specificity
    ab75831 detects eIF4EBP1 phosphorylated at threonine 70.
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 70 of human eIF4EBP1.

  • Positive control
    • 293T cell lysates treated with FBS.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab75831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • Sequence similarities
      Belongs to the eIF4E-binding protein family.
    • Post-translational
      Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all


    • All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831) at 1/500 dilution

      Lane 1 : 293T cell lysates un-treated
      Lane 2 : 293T cell lysates treated with FBS

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 13 kDa
      Observed band size: 17 kDa (why is the actual band size different from the predicted?)


    This product has been referenced in:
    • Liu Y  et al. Effects of bufalin on the mTOR/p70S6K pathway and apoptosis in esophageal squamous cell carcinoma in nude mice. Int J Mol Med 40:357-366 (2017). Read more (PubMed: 28656204) »
    • Li S  et al. Clinical and genetic analysis of tuberous sclerosis complex-associated renal angiomyolipoma in Chinese pedigrees. Oncol Lett 14:7085-7090 (2017). Read more (PubMed: 29344138) »

    See all 2 Publications for this product

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