- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-eIF4G1 antibody
See all eIF4G1 primary antibodies -
DescriptionRabbit polyclonal to eIF4G1
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Rat, Human, African green monkey
Predicted to work with: Mouse, Rabbit, Horse, Hamster, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide (Human) - which represents a portion of human eukaryotic translation InitiationFactor 4 Gamma 1 encoded in part by exons 10 and 11.
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Positive control
- Rat liver lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
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Concentration information loading... -
Purification notesAffinity purified using the immunising peptide immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab2609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/1000. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 220 kDa). EIF4G is more susceptible to degradation compared to other proteins, especially from some tissue sources such as liver. This is true even when tissue is stored at frozen. SDS-PAGE sample buffer may improve the stability, but samples that are stored frozen may show degradation bands that have been described in the literature.Therefore if multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody. | |
| IHC-P | Use a concentration of 4 µg/ml. | |
| ICC/IF | 1/500. |
Target
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FunctionComponent of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
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Involvement in diseaseDefects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
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Sequence similaritiesBelongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain. -
Post-translational
modificationsPhosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription. - Information by UniProt
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Database links
- Entrez Gene: 1981 Human
- Entrez Gene: 208643 Mouse
- Entrez Gene: 287986 Rat
- Omim: 600495 Human
- SwissProt: Q04637 Human
- SwissProt: Q6NZJ6 Mouse
- SwissProt: P41110 Rabbit
- SwissProt: Q4G001 Rat
see all -
Alternative names
- DKFZp686A1451 antibody
- eIF 4 gamma 1 antibody
- eIF 4G 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 antibody (ab2609)ab2609 (4µg/ml) staining eIF4G1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm of the intestinal cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunoprecipitation - Anti-eIF4G1 antibody (ab2609)All lanes : Anti-eIF4G1 antibody (ab2609) at 1 µg/ml
Lane 1 : HeLa cells immunoprecipitated by ab2609 at 6 µg per reaction
Lane 2 : HeLa cells immunoprecipitated by control IgG at 6 µg per reaction
Exposure time: 10 seconds -
Western blot - Anti-eIF4G1 antibody (ab2609)50
50µg (lane 1) and 150µg (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1:1000 (A), 1:5000 (B) and 1:10000 (C) dilution by ECL.µ g (lane 1) and 150µ g (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1:1000 (A), 1:5000 (B) and 1:10000 (C) dilution by ECL. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4G1 antibody (ab2609)ICC/IF image of ab2609 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2609, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Wu H et al. N-Terminal Domain of Feline Calicivirus (FCV) Proteinase-Polymerase Contributes to the Inhibition of Host Cell Transcription. Viruses 8:N/A (2016). Read more (PubMed: 27447663) »
- Van Rechem C et al. Lysine demethylase KDM4A associates with translation machinery and regulates protein synthesis. Cancer Discov 5:255-63 (2015). WB ; Human . Read more (PubMed: 25564516) »
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