Product nameAnti-eIF4G1 antibody
See all eIF4G1 primary antibodies
DescriptionRabbit polyclonal to eIF4G1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit
Synthetic peptide within Human eIF4G1 aa 250-300. The exact sequence is proprietary.
Database link: Q04637
- WB: HeLa whole cell lysate; IP: HeLa whole cell lysate; IHC-P: Human ovarian carcinoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000.|
FunctionComponent of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
Involvement in diseaseDefects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
Sequence similaritiesBelongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain.
modificationsPhosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
- Information by UniProt
- DKFZp686A1451 antibody
- eIF 4 gamma 1 antibody
- eIF 4G 1 antibody
All lanes : Anti-eIF4G1 antibody (ab264232) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Exposure time: 3 seconds
Immunohistochemical analysis of FFPE human ovarian carcinoma tissue section labeling eIF4G1 with ab264232 at 1/200 dilution. Detection: DAB.
eIF4G1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264232 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264232 at 1 µg/ml.
Lane 1: ab264232 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 1 sec.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264232 has not yet been referenced specifically in any publications.