Overview

  • Product name

    Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free
    See all eIF4G1 primary antibodies
  • Description

    Rabbit monoclonal [SP351] to eIF4G1 (phospho S1108) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF4G1 (phospho S1108). The exact sequence is proprietary.
    Database link: Q04637

  • Positive control

    • IHC-P: Human lung carcinoma, ovarian carcinoma, and bladder transitional cell carcinoma tissue.
  • General notes

    Ab242419 is the carrier-free version of ab223502. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242419 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab242419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary Antibody Incubation: 10 minutes at room temperature.

Target

  • Function

    Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
  • Involvement in disease

    Defects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
  • Sequence similarities

    Belongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • Post-translational
    modifications

    Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686A1451 antibody
    • eIF 4 gamma 1 antibody
    • eIF 4G 1 antibody
    • eIF 4G1 antibody
    • eIF-4-gamma 1 antibody
    • eIF-4G 1 antibody
    • eIF-4G1 antibody
    • EIF4 gamma antibody
    • EIF4F antibody
    • EIF4G antibody
    • EIF4G1 antibody
    • EIF4GI antibody
    • Eukaryotic translation initiation factor 4 gamma 1 antibody
    • IF4G1_HUMAN antibody
    • p220 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling eIF4G1 with Purified ab223502 at 1/100 dilution (1.20 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223502)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling eIF4G1 with Purified ab223502 at 1/100 dilution (1.20 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223502)
  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for eIF4G1 (phospho S1108) with ab223502 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223502).

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for eIF4G1 (phospho S1108) with ab223502 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223502).

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for eIF4G1 (phospho S1108) with ab223502 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223502).

References

ab242419 has not yet been referenced specifically in any publications.

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