Product nameAnti-eIF4GII antibody
See all eIF4GII primary antibodies
DescriptionRabbit polyclonal to eIF4GII
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83330 is purified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titer in peptide based assay is 1/1562500.
FunctionProbable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4G family.
Contains 5 HEAT repeats.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain.
modificationsFollowing infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
- Information by UniProt
- eIF-4-gamma 3 antibody
- eIF-4-gamma II antibody
- eIF-4G 3 antibody
Anti-eIF4GII antibody (ab83330) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 177 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: <40 kDa. We are unsure as to the identity of these extra bands.