Anti-eIF4GII antibody (ab83330)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-eIF4GII antibody
See all eIF4GII primary antibodies -
Description
Rabbit polyclonal to eIF4GII -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
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Immunogen
Synthetic peptide corresponding to a region within amino acids 1152-1201 (
MRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRES
) of human EIF4G3 (NP_003751). -
Positive control
- HeLa whole cell lysate (ab150035).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab83330 is purified by peptide affinity chromatography method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. Titer in peptide based assay is 1/1562500. |
Target
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Function
Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4G family.
Contains 5 HEAT repeats.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain. -
Post-translational
modificationsFollowing infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription. - Information by UniProt
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Database links
- Entrez Gene: 8672 Human
- Entrez Gene: 230861 Mouse
- Entrez Gene: 298573 Rat
- Omim: 603929 Human
- SwissProt: O43432 Human
- SwissProt: Q80XI3 Mouse
- Unigene: 467084 Human
- Unigene: 732241 Human
see all -
Alternative names
- eIF-4-gamma 3 antibody
- eIF-4-gamma II antibody
- eIF-4G 3 antibody
see all
Images
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Western blot - Anti-eIF4GII antibody (ab83330)Anti-eIF4GII antibody (ab83330) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 177 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: <40 kDa. We are unsure as to the identity of these extra bands.
12% gel
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wu H et al. N-Terminal Domain of Feline Calicivirus (FCV) Proteinase-Polymerase Contributes to the Inhibition of Host Cell Transcription. Viruses 8: (2016). WB . Read more (PubMed: 27447663) »
