Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF4GII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1152-1201 (

    MRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRES

    ) of human EIF4G3 (NP_003751).

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab83330 is purified by peptide affinity chromatography method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based assay is 1/1562500.

Target

  • Function

    Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
  • Sequence similarities

    Belongs to the eukaryotic initiation factor 4G family.
    Contains 5 HEAT repeats.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • Post-translational
    modifications

    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • Database links

  • Alternative names

    • eIF-4-gamma 3 antibody
    • eIF-4-gamma II antibody
    • eIF-4G 3 antibody
    • eIF4G 3 antibody
    • EIF4G3 antibody
    • eIF4GII antibody
    • Eukaryotic translation initiation factor 4 gamma 3 antibody
    • eukaryotic translation initiation factor 4 gamma, 3 antibody
    • eukaryotic translation initiation factor-4 gamma, 3 antibody
    • IF4G3_HUMAN antibody
    see all

Images

  • Anti-eIF4GII antibody (ab83330) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 177 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?
    Additional bands at: <40 kDa. We are unsure as to the identity of these extra bands.



    12% gel

References

This product has been referenced in:

  • Wu H  et al. N-Terminal Domain of Feline Calicivirus (FCV) Proteinase-Polymerase Contributes to the Inhibition of Host Cell Transcription. Viruses 8: (2016). WB . Read more (PubMed: 27447663) »
See 1 Publication for this product

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