Anti-eIF4GII antibody - N-terminal (ab155482)
Key features and details
- Rabbit polyclonal to eIF4GII - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4GII antibody - N-terminal -
Description
Rabbit polyclonal to eIF4GII - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to a region within N-terminal amino acids 1-203 of Human eIF4GII (Uniprot: O43432).
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Positive control
- Recombinant Human eIF4GII protein (ab132478) can be used as a positive control in WB. 293T, A431, HeLa, JC, BCL-1 and HepG2 whole cell lysates. Human AGS xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 177 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 177 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4G family.
Contains 5 HEAT repeats.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain. -
Post-translational
modificationsFollowing infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription. - Information by UniProt
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Database links
- Entrez Gene: 8672 Human
- Entrez Gene: 230861 Mouse
- Entrez Gene: 298573 Rat
- Omim: 603929 Human
- SwissProt: O43432 Human
- SwissProt: Q80XI3 Mouse
- Unigene: 467084 Human
- Unigene: 732241 Human
see all -
Alternative names
- eIF-4-gamma 3 antibody
- eIF-4-gamma II antibody
- eIF-4G 3 antibody
see all
Images
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All lanes : Anti-eIF4GII antibody - N-terminal (ab155482) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 177 kDa
5% SDS PAGE -
All lanes : Anti-eIF4GII antibody - N-terminal (ab155482) at 1/5000 dilution
Lane 1 : Mouse JC whole cell lysate
Lane 2 : Mouse BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 177 kDa
5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human AGS xenograft tissue, labeling eIF4GII using ab155482 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155482 has not yet been referenced specifically in any publications.