• Product name

    Anti-eIF4H antibody
  • Description

    Rabbit polyclonal to eIF4H
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    A region between residue 198 and 248 of human eIF4H using the numbering given in entry NP_071496.1

  • Positive control

    • HeLa cells lysate 293T cells lysate
  • General notes

    Shelf Life: 1 year from date of receipt


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity purified using an epitope specific to eIF4H immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab86179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
    • Tissue specificity

      The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
    • Involvement in disease

      Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities

      Contains 1 RRM (RNA recognition motif) domain.
    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt
    • Database links

    • Alternative names

      • AU018978 antibody
      • D5Ertd355e antibody
      • E430026L18Rik antibody
      • Ef4h antibody
      • eIF 4H antibody
      • eIF-4H antibody
      • EIF4H antibody
      • Eukaryotic translation initiation factor 4H antibody
      • IF4H_HUMAN antibody
      • KIAA0038 antibody
      • WBSCR1 antibody
      • Williams Beuren syndrome chromosomal region 1 protein homolog antibody
      • Williams Beuren syndrome chromosome region 1 antibody
      • Williams-Beuren syndrome chromosomal region 1 protein antibody
      • Williams-Beuren syndrome chromosome region 1 antibody
      • WSCR1 antibody
      see all


    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 27 kDa

      Exposure time: 3 minutes


    ab86179 has not yet been referenced specifically in any publications.

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