Key features and details
- Rabbit polyclonal to eIF4H
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4H antibody
DescriptionRabbit polyclonal to eIF4H
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
A region between residue 198 and 248 of human eIF4H using the numbering given in entry NP_071496.1
- HeLa cells lysate 293T cells lysate
Storage instructionsShipped at 4°C. Store at -20°C. The product can be stored for up to 12 months.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to eIF4H immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Our Abpromise guarantee covers the use of ab86179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 27 kDa.|
FunctionStimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
Tissue specificityThe short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
Involvement in diseaseNote=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- AU018978 antibody
- D5Ertd355e antibody
- E430026L18Rik antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 3 minutes
ab86179 has not yet been referenced specifically in any publications.