Product nameAnti-EIF5 antibody
See all EIF5 primary antibodies
DescriptionRabbit polyclonal to EIF5
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
Synthetic peptide, corresponding to a region between residues 100 and 150 of Human EIF5 (NP_892116.2)
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85913 was affinity purified using an epitope specific to EIF5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
FunctionCatalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Sequence similaritiesBelongs to the eIF-2-beta/eIF-5 family.
Contains 1 W2 domain.
- Information by UniProt
- 2810011H21Rik antibody
- D12Ertd549e antibody
- EIF 5 antibody
All lanes : Anti-EIF5 antibody (ab85913) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 49 kDa
Observed band size: 49 kDa
This product has been referenced in:
- Loughran G et al. Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection. PLoS One 13:e0192648 (2018). Read more (PubMed: 29470543) »
- Tzani I et al. Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform. Open Biol 6:N/A (2016). Read more (PubMed: 27249819) »