Recombinant
RabMAb

Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Overview

  • Product name
    Anti-EIF5 antibody [EPR12140(B)]
    See all EIF5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12140(B)] to EIF5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EIF5 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P55010

  • Positive control
    • K562, HeLa and HepG2 cell lysates; Human colonic carcinoma and pancreas tissues; K562 and HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12140(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • Flow cytometric analysis of permeabilized K562 cells labeling EIF5 with ab170915 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    • Immunofluorescence analysis of HepG2 cells labeling EIF5 with ab170915 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling EIF5 with ab170915 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling EIF5 with ab170915 at 1/50 dilution.

    • All lanes : Anti-EIF5 antibody [EPR12140(B)] (ab170915) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 49 kDa

    References

    ab170915 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170915.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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