Recombinant Anti-EIF5 antibody [EPR12140(B)] (ab170915)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12140(B)] to EIF5
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EIF5 antibody [EPR12140(B)]
See all EIF5 primary antibodies -
Description
Rabbit monoclonal [EPR12140(B)] to EIF5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human EIF5 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P55010 -
Positive control
- K562, HeLa and HepG2 whole cell lysate (ab7900); Human colonic carcinoma and pancreas tissues; K562 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12140(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). -
Sequence similarities
Belongs to the eIF-2-beta/eIF-5 family.
Contains 1 W2 domain. - Information by UniProt
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Database links
- Entrez Gene: 1983 Human
- Entrez Gene: 100047658 Mouse
- Entrez Gene: 217869 Mouse
- Entrez Gene: 56783 Rat
- Omim: 601710 Human
- SwissProt: P55010 Human
- SwissProt: P59325 Mouse
- SwissProt: Q07205 Rat
see all -
Alternative names
- 2810011H21Rik antibody
- D12Ertd549e antibody
- EIF 5 antibody
see all
Images
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All lanes : Anti-EIF5 antibody [EPR12140(B)] (ab170915) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 49 kDa -
Intracellular flow cytometric analysis of permeabilized K562 cells labeling EIF5 with ab170915 at 1/100 dilution (red) compared toa rabbit IgG negative control (green).
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Immunofluorescence analysis of HepG2 cells labeling EIF5 with ab170915 at 1/100 dilution (green). DAPI nuclear staining (blue).
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling EIF5 with ab170915 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling EIF5 with ab170915 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab170915 has been referenced in 1 publication.
- Karlina R et al. Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice. Life Sci Alliance 4:N/A (2021). PubMed: 33257475