Product nameAnti-EIF5 antibody [EPR12140(B)]
See all EIF5 primary antibodies
DescriptionRabbit monoclonal [EPR12140(B)] to EIF5
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EIF5 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P55010
- K562, HeLa and HepG2 whole cell lysate (ab7900); Human colonic carcinoma and pancreas tissues; K562 and HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Sequence similaritiesBelongs to the eIF-2-beta/eIF-5 family.
Contains 1 W2 domain.
- Information by UniProt
- 2810011H21Rik antibody
- D12Ertd549e antibody
- EIF 5 antibody
All lanes : Anti-EIF5 antibody [EPR12140(B)] (ab170915) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 49 kDa
Flow cytometric analysis of permeabilized K562 cells labeling EIF5 with ab170915 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Immunofluorescence analysis of HepG2 cells labeling EIF5 with ab170915 at 1/100 dilution (green). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling EIF5 with ab170915 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling EIF5 with ab170915 at 1/50 dilution.
ab170915 has not yet been referenced specifically in any publications.