Key features and details
- Mouse monoclonal [4E10F6] to eIF5A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-eIF5A antibody [4E10F6]
See all eIF5A primary antibodies
DescriptionMouse monoclonal [4E10F6] to eIF5A
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
Recombinant full length protein corresponding to Human eIF5A aa 1-154. (Purified from E.coli).
MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGK HGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLS LLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK AMAK
Database link: P63241
- Recombinant Human eIF5A protein; eIF5A (aa1-154)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human colon cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab204939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 16 kDa.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.|
FunctionmRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Tissue specificityExpressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
Sequence similaritiesBelongs to the eIF-5A family.
modificationseIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Cellular localizationCytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
- Information by UniProt
- eIF 4D antibody
- eIF 5A 1 antibody
- EIF 5A antibody
Anti-eIF5A antibody [4E10F6] (ab204939) at 1/500 dilution + Recombinant Human eIF5A protein
Predicted band size: 16 kDa
Expected MWt is 42.8 kDa.
All lanes : Anti-eIF5A antibody [4E10F6] (ab204939) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : eIF5A (aa1-154)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 16 kDa
Flow cytometric analysis of HeLa cells labeling eIF5A with ab204939 at 1/200 dilution (green), compared to a negative control (red).
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling eIF5A with ab204939 at 1/200 dilution with DAB staining.
ab204939 has not yet been referenced specifically in any publications.