Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF5A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF5A aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate, Human lung carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab32014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 18 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
    • Tissue specificity

      Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
    • Sequence similarities

      Belongs to the eIF-5A family.
    • Post-translational
      modifications

      eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • Cellular localization

      Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • Database links

    • Alternative names

      • eIF 4D antibody
      • eIF 5A 1 antibody
      • EIF 5A antibody
      • eIF 5A1 antibody
      • eIF-4D antibody
      • eIF-5A antibody
      • eIF-5A-1 antibody
      • eIF-5A1 antibody
      • eIF4D antibody
      • eif5a antibody
      • EIF5A1 antibody
      • eIF5AI antibody
      • Eukaryotic initiation factor 5A antibody
      • Eukaryotic initiation factor 5A isoform 1 antibody
      • Eukaryotic translation initiation factor 5A 1 antibody
      • Eukaryotic translation initiation factor 5A antibody
      • Eukaryotic translation initiation factor 5A-1 antibody
      • IF5A1_HUMAN antibody
      • MGC104255 antibody
      • MGC99547 antibody
      • Rev binding factor antibody
      • Rev-binding factor antibody
      • uORF A antibody
      • uORF antibody
      see all

    Images

    • ab32014, at a 1/100 dilution, staining human eIF5A in lung carcinoma by Immunohistochemistry, Paraffin embedded tissue
    • Anti-eIF5A antibody (ab32014) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    References

    This product has been referenced in:

    • Mancone C  et al. Iron overload down-regulates the expression of the HIV-1 Rev cofactor eIF5A in infected T lymphocytes. Proteome Sci 15:18 (2017). Read more (PubMed: 28785172) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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