• Product name

    Anti-eIF5A antibody [EP527Y]
    See all eIF5A primary antibodies
  • Description

    Rabbit monoclonal [EP527Y] to eIF5A
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF5A aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899). IHC-P: human urinary bladder carcinoma.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
    • Tissue specificity

      Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
    • Sequence similarities

      Belongs to the eIF-5A family.
    • Post-translational

      eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • Cellular localization

      Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • Database links

    • Alternative names

      • eIF 4D antibody
      • eIF 5A 1 antibody
      • EIF 5A antibody
      • eIF 5A1 antibody
      • eIF-4D antibody
      • eIF-5A antibody
      • eIF-5A-1 antibody
      • eIF-5A1 antibody
      • eIF4D antibody
      • eif5a antibody
      • EIF5A1 antibody
      • eIF5AI antibody
      • Eukaryotic initiation factor 5A antibody
      • Eukaryotic initiation factor 5A isoform 1 antibody
      • Eukaryotic translation initiation factor 5A 1 antibody
      • Eukaryotic translation initiation factor 5A antibody
      • Eukaryotic translation initiation factor 5A-1 antibody
      • IF5A1_HUMAN antibody
      • MGC104255 antibody
      • MGC99547 antibody
      • Rev binding factor antibody
      • Rev-binding factor antibody
      • uORF A antibody
      • uORF antibody
      see all


    • Anti-eIF5A antibody [EP527Y] (ab32407) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    • Ab32407, at a dilution of 1/250, staining eIF5A in paraffin embedded human urinary bladder carcinoma tissue sections by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Overlay histogram showing Jurkat cells stained with ab32407 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32407, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


    This product has been referenced in:

    • Saxena R  et al. Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants. PLoS One 10:e0122994 (2015). WB . Read more (PubMed: 25874870) »
    • Scuoppo C  et al. A tumour suppressor network relying on the polyamine-hypusine axis. Nature 487:244-8 (2012). IHC, WB . Read more (PubMed: 22722845) »
    See all 2 Publications for this product

    Customer reviews and Q&As


    We have tested ab32407 in IP and in our hands we could not get this to work hence why we have stated on the datasheet that this antibody is unsuitable for use in this application.

    If you would like to use an Anti-eIF5A antibody guaranteed to work in IP, I would recommend Anti-eIF5A antibody [EP526Y] (ab32443) which is very similar to ab32407 but this antibody has actually been seen to give good results in IP and is therefore covered by our Abpromise guarantee.

    Read More

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