Recombinant Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)
![Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)](https://www.abcam.com/ps/products/126/ab126735/Images/ab126735-140717-anti-eif5a2-antibody-epr7411-6-western-blot.jpg "Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit Monoclonal [EPR7411-6] to eIF5A2 + eIF5A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-eIF5A2 + eIF5A antibody [EPR7411-6]
See all eIF5A2+eIF5A primary antibodies -
Description
Rabbit monoclonal [EPR7411-6] to eIF5A2 + eIF5A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human eIF5A2 aa 1-100 (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
Database link: Q9GZV4 -
Positive control
- WB: 293T, HeLa, A431, Caco 2 and HepG2 whole cell lysate (ab7900); IHC-P: Human ovarian carcinoma tissue; Flow Cyt: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7411-6 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Cellular localization
eIF5A2: Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. eIF5A: Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. -
Database links
- Entrez Gene: 1984 Human
- Entrez Gene: 56648 Human
- Entrez Gene: 208691 Mouse
- Entrez Gene: 276770 Mouse
- Entrez Gene: 287444 Rat
- Entrez Gene: 310261 Rat
- Omim: 600187 Human
- Omim: 605782 Human
see all
Images
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Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : CaCo 2 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa -
![Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)](https://www.abcam.com/ps/products/126/ab126735/Images/ab126735-195339-anti-eif5a2-antibody-epr7411-6-flow-cytometry.jpg)
Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)Overlay histogram showing HeLa cells stained with ab126735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126735, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)](https://www.abcam.com/ps/products/126/ab126735/Images/ab126735-140716-anti-eif5a2-antibody-epr7411-6-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)ab126735 at 1/100 dilution, labeling eIF5A2 + eIF5A in Formalin fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)](https://www.abcam.com/ps/products/126/ab126735/Images/ab126735-451089-anti-eif5a2-eif5a-antibody-epr7411-6-western-blot-recombinant-human.jpg)
Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/2000 dilution
Lane 1 :Recombinant Human eIF5A protein (ab87457)
Lane 2 :Recombinant Human eIF5A2 protein (ab99140)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 15,20 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer: 5% NFDM/TBST
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![OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)](https://www.abcam.com/ps/products/126/ab126735/Images/ab126735-140715-anti-eif5a2-antibody-epr7411-6.jpg)
OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) -
![Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)](https://www.abcam.com/ps/products/126/ab126735/Images/ab126735-5-benefits-of-recombinant-antibodies.png)
Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)
Protocols
Datasheets and documents
References (3)
ab126735 has been referenced in 3 publications.
- Wu Y et al. Chimeric peptide supramolecular nanoparticles for plectin-1 targeted miRNA-9 delivery in pancreatic cancer. Theranostics 10:1151-1165 (2020). PubMed: 31938057
- Meng QB et al. Overexpression of eukaryotic translation initiation factor 5A2 (EIF5A2) correlates with cell aggressiveness and poor survival in gastric cancer. PLoS One 10:e0119229 (2015). PubMed: 25793713
- Qin X et al. Inhibition of eIF5A results in aberrant uterine natural killer cell function and embryo loss in mice. Am J Reprod Immunol 71:229-40 (2014). PubMed: 24382123
