RecombinantRabMAb

Recombinant Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

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Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
  • Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
  • Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7411-6] to eIF5A2 + eIF5A - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free
    See all eIF5A2 + eIF5A primary antibodies

  • Description

    Rabbit monoclonal [EPR7411-6] to eIF5A2 + eIF5A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human eIF5A2 + eIF5A aa 1-100 (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
    Database link: Q9GZV4

  • General notes

    Ab248154 is the carrier-free version of ab126735. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248154 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

Images

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Caco-2 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa



    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Flow Cytometry - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

    Overlay histogram showing HeLa cells stained with ab126735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126735, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

    ab126735 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/2000 dilution

    Lane 1 : Recombinant Human eIF5A protein (ab87457)
    Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 15,20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab126735, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    This data was developed using ab126735, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)
    Anti-eIF5A2 + eIF5A antibody [EPR7411-6] - BSA and Azide free (ab248154)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248154? Please let us know so that we can cite the reference in this datasheet.

ab248154 has not yet been referenced specifically in any publications.

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