Anti-eIF5A2 antibody (ab168122)
- Datasheet
- References
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Overview
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Product nameAnti-eIF5A2 antibody
See all eIF5A2 primary antibodies -
DescriptionMouse polyclonal to eIF5A2
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Chimpanzee, Rhesus monkey, Chinese hamster
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Immunogen
Full length protein, corresponding to amino acids 1-153 of Human eIF5A2 (NP_065123.1)
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Positive control
- eIF5A2 transfected 293T cell lysate; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionmRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
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Tissue specificityExpressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
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Sequence similaritiesBelongs to the eIF-5A family.
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Post-translational
modificationseIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine). -
Cellular localizationCytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
- Information by UniProt
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Database links
- Entrez Gene: 744869 Chimpanzee
- Entrez Gene: 506307 Cow
- Entrez Gene: 488164 Dog
- Entrez Gene: 56648 Human
- Entrez Gene: 208691 Mouse
- Entrez Gene: 310261 Rat
- Entrez Gene: 695647 Rhesus monkey
- Omim: 605782 Human
see all -
Alternative names
- 9630038B20 antibody
- eIF 5A 2 antibody
- eIF 5A2 antibody
see all
Images
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Western blot - Anti-eIF5A2 antibody (ab168122)All lanes : Anti-eIF5A2 antibody (ab168122) at 1 µg/ml
Lane 1 : eIF5A2 Transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG
Predicted band size: 17 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 antibody (ab168122)Immunofluorescence of HeLa cells labeling eIF5A2 using ab168122 at 10 µg/ml.
Datasheets and documents
References
ab168122 has not yet been referenced specifically in any publications.
