Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF5A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human eIF5A2 (internal sequence). The exact sequence is proprietary.
    Database link: Q9GZV4

  • Positive control

    • WB: HEK-293T, A431 and HeLa whole cell lysates. IHC-P: Human gastric cancer tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
  • Tissue specificity

    Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
  • Sequence similarities

    Belongs to the eIF-5A family.
  • Post-translational
    modifications

    eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • Cellular localization

    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9630038B20 antibody
    • eIF 5A 2 antibody
    • eIF 5A2 antibody
    • eIF-4D antibody
    • eIF-5A-2 antibody
    • eIF-5A2 antibody
    • EIF5A 2 antibody
    • EIF5A2 antibody
    • eIF5AII antibody
    • Eukaryotic initiation factor 5A isoform 2 antibody
    • Eukaryotic translation initiation factor 5A-2 antibody
    • Eukaryotic translation initiation factor 5A2 antibody
    • IF5A2_HUMAN antibody
    see all

Images

  • All lanes : Anti-eIF5A2 antibody (ab227537) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 17 kDa



    15% SDS-PAGE gel.

  • Paraffin-embedded human gastric cancer tissue stained for eIF5A2 with ab227537 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for eIF5A2 (green) using ab227537 at 1/500 dilution in ICC/IF.

    Alpha-tubulin filaments are labelled with an anti-alpha-Tubulin antibody (red).

References

ab227537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227537.
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