Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7411-105] to eIF5A2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-eIF5A2 antibody [EPR7411-105]
See all eIF5A2 primary antibodies
DescriptionRabbit monoclonal [EPR7411-105] to eIF5A2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to residues near the N terminal of Human eIF5A2 (UniProt: Q9GZV4).
- HeLa, SW480, RAW 264.7, NIH 3T3 and 293T cell lysates; permeabilized HeLa cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 17 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionmRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
Tissue specificityExpressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
Sequence similaritiesBelongs to the eIF-5A family.
modificationseIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Cellular localizationCytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
- Information by UniProt
- 9630038B20 antibody
- eIF 5A 2 antibody
- eIF 5A2 antibody
All lanes : Anti-eIF5A2 antibody [EPR7411-105] (ab150403) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : RAW264.7 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Flow cytometric analysis of permeabilized HeLa cells labelling elF5A2 with ab150403 at 1/10 dilution (red); compared to a negative rabbit IgG control (green).
ab150403 has not yet been referenced specifically in any publications.