Overview

  • Product name

    Anti-eIF5A2 antibody [EPR7411-105]
    See all eIF5A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7411-105] to eIF5A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues near the N terminal of Human eIF5A2 (UniProt: Q9GZV4).

  • Positive control

    • HeLa, SW480, RAW 264.7, NIH 3T3 and 293T cell lysates; permeabilized HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
    • Tissue specificity

      Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
    • Sequence similarities

      Belongs to the eIF-5A family.
    • Post-translational
      modifications

      eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • Cellular localization

      Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • Database links

    • Alternative names

      • 9630038B20 antibody
      • eIF 5A 2 antibody
      • eIF 5A2 antibody
      • eIF-4D antibody
      • eIF-5A-2 antibody
      • eIF-5A2 antibody
      • EIF5A 2 antibody
      • EIF5A2 antibody
      • eIF5AII antibody
      • Eukaryotic initiation factor 5A isoform 2 antibody
      • Eukaryotic translation initiation factor 5A-2 antibody
      • Eukaryotic translation initiation factor 5A2 antibody
      • IF5A2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-eIF5A2 antibody [EPR7411-105] (ab150403) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : RAW264.7 cell lysate
      Lane 4 : NIH 3T3 cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labelling elF5A2 with ab150403 at 1/10 dilution (red); compared to a negative rabbit IgG control (green).

    References

    ab150403 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150403.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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