Overview

  • Product name

    Anti-eIF5A2 antibody [EPR7412-47]
    See all eIF5A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7412-47] to eIF5A2
  • Host species

    Rabbit
  • Specificity

    The antibody may react with not only A2 but also A1.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF5A2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HepG2 and Caco 2 cell lysates; Human ovarian carcinoma tissue; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
    • Tissue specificity

      Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
    • Sequence similarities

      Belongs to the eIF-5A family.
    • Post-translational
      modifications

      eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • Cellular localization

      Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • Database links

    • Alternative names

      • 9630038B20 antibody
      • eIF 5A 2 antibody
      • eIF 5A2 antibody
      • eIF-4D antibody
      • eIF-5A-2 antibody
      • eIF-5A2 antibody
      • EIF5A 2 antibody
      • EIF5A2 antibody
      • eIF5AII antibody
      • Eukaryotic initiation factor 5A isoform 2 antibody
      • Eukaryotic translation initiation factor 5A-2 antibody
      • Eukaryotic translation initiation factor 5A2 antibody
      • IF5A2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-eIF5A2 antibody [EPR7412-47] (ab126733) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa

    • ab126733 at 1/100 dilution, staining eIF5A2 in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab126733, at 1/100 dilution, staining eLF5A2 in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126733 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us. The antibody ab150439 is tested and is very specific for eIF5A-2. Regarding eIF5A-1 (otherwise known as eIF5A) many of these antibodies have some amount of cross-reactivity, although one predicted to be least likely to cross-react is ab106782, based on homology with the immunogen.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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