Recombinant Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF5A2 + eIF5A antibody [EPR7412-50]
See all eIF5A2 + eIF5A primary antibodies -
Description
Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human eIF5A2 + eIF5A aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
Database link: Q9GZV4 -
Positive control
- Recombinant Human eIF5A2 protein (ab99140) can be used as a positive control in WB. HepG2, HeLa, 293T and Raji cell lysates; Human colon adenocarcinoma and testis tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7412-50 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
eIF5A2: Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. eIF5A: Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. -
Database links
- Entrez Gene: 1984 Human
- Entrez Gene: 56648 Human
- Entrez Gene: 208691 Mouse
- Entrez Gene: 276770 Mouse
- Entrez Gene: 287444 Rat
- Entrez Gene: 310261 Rat
- Omim: 600187 Human
- Omim: 605782 Human
see all
Images
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All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled antibody at 1/2000 dilution
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution
Lane 1 :Recombinant Human eIF5A protein (ab87457)
Lane 2 :Recombinant Human eIF5A2 protein (ab99140)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 15,20 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking buffer: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150439 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150439 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150439 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab150439 has been referenced in 12 publications.
- Xu J et al. LINC00518 affects the proliferation, invasion and migration of cutaneous malignant melanoma cells via miR-526b-3p/EIF5A2 axis. Acta Biochim Pol 69:101-111 (2022). PubMed: 35189053
- Wang K et al. EIF5A2 enhances stemness of epithelial ovarian cancer cells via a E2F1/KLF4 axis. Stem Cell Res Ther 12:186 (2021). PubMed: 33726845
- Bao Y et al. Overexpression of microRNA-9 enhances cisplatin sensitivity in hepatocellular carcinoma by regulating EIF5A2-mediated epithelial-mesenchymal transition. Int J Biol Sci 16:827-837 (2020). PubMed: 32071552
- Capella Roca B et al. Altered gene expression in CHO cells following polyamine starvation. Biotechnol Lett 42:927-936 (2020). PubMed: 32078082
- Ai J et al. Long non-coding RNA GAS6-AS1 acts as a ceRNA for microRNA-585, thereby increasing EIF5A2 expression and facilitating hepatocellular carcinoma oncogenicity. Cell Cycle 19:742-757 (2020). PubMed: 32089066