Overview

  • Product name

  • Description

    Rabbit polyclonal to ELAC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human ELAC2 (internal sequence). The exact sequence is proprietary.
    Database link: Q9BQ52

  • Positive control

    • WB: Jurkat, Raji and NCI-H929 whole cell extracts. IP: Jurkat whole cell extract. IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.
  • Tissue specificity

    Widely expressed. Highly expressed in heart, placenta, liver, skeletal muscle, kidney, pancreas, testis and ovary. Weakly expressed in brain, lung, spleen, thymus, prostate, small intestine, colon and leukocytes.
  • Involvement in disease

    Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
  • Sequence similarities

    Belongs to the RNase Z family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • COXPD17 antibody
    • elaC homolog 2 (E. coli) antibody
    • ElaC homolog protein 2 antibody
    • elaC ribonuclease Z 2 antibody
    • elaC, E. coli, homolog of, 2 antibody
    • ELAC2 antibody
    • ELC2 antibody
    • FLJ10530 antibody
    • FLJ36693 antibody
    • FLJ42848 antibody
    • Heredity prostate cancer protein 2 antibody
    • HPC2 antibody
    • putative prostate cancer susceptibility protein HPC2/ELAC2 antibody
    • Ribonuclease Z 2 antibody
    • Ribonuclease Z, long form antibody
    • Ribonuclease Z2 antibody
    • RNase Z 2 antibody
    • RNase Z2 antibody
    • RNase ZL antibody
    • RNZ2_HUMAN antibody
    • tRNA 3 endonuclease 2 antibody
    • tRNase Z (long form) antibody
    • tRNase Z 2 antibody
    • tRNase Z2 antibody
    • Zinc phosphodiesterase ELAC protein 2 antibody
    see all

Images

  • All lanes : Anti-ELAC2 antibody (ab226781) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 92 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human breast carcinoma tissue stained for ELAC2 using ab226781 at 1/500 dilution in immunohistochemical analysis.

  • ELAC2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab226781. Western blot was performed from the immunoprecipitate using ab226781.

    Lane 1: Jurkat whole cell extract.

    Lane 2: Control IgG IP in Jurkat whole cell extract.

    Lane 3: ab226781 IP in Jurkat whole cell extract.

References

ab226781 has not yet been referenced specifically in any publications.

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