Product nameAnti-Elafin/ESI antibody
See all Elafin/ESI primary antibodies
DescriptionRabbit polyclonal to Elafin/ESI
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Elafin/ESI (internal sequence).
(Peptide available as
Previously labelled as Elafin/Skalp.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab46774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Predicted molecular weight: 13 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionNeutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Sequence similaritiesContains 1 WAP domain.
DomainConsists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.
- Information by UniProt
- Cementoin antibody
- ELAF_HUMAN antibody
- Elafin antibody
All lanes : Anti-Elafin/ESI antibody (ab46774) at 1/1000 dilution
Lane 1 : recombinant Human Elafin at 0.05 µg
Lane 2 : recombinant Human Elafin at 0.01 µg
Lane 3 : recombinant Human Elafin at 0.001 µg
Predicted band size: 13 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible dimer)
ICC/IF image of ab46774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46774, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Zhang L et al. Overexpression of MTA1 inhibits the metastatic ability of ZR-75-30 cells in vitro by promoting MTA2 degradation. Cell Commun Signal 17:4 (2019). Read more (PubMed: 30642362) »
- Harman RM et al. Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds. Stem Cell Res Ther 8:157 (2017). Read more (PubMed: 28676123) »