Recombinant Anti-Elafin/ESI antibody [EPR14631] - BSA and Azide free (ab236135)
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Overview
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Product name
Anti-Elafin/ESI antibody [EPR14631] - BSA and Azide free
See all Elafin/ESI primary antibodies -
Description
Rabbit monoclonal [EPR14631] to Elafin/ESI - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Elafin/ESI aa 1-100. The exact sequence is proprietary.
Database link: P19957 -
Positive control
- WB: A431 cell lysate.
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General notes
ab236135 is the carrier-free version of ab184972 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab236135 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Elafin/Skalp.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14631 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 12, 14 kDa (predicted molecular weight: 12 kDa). |
Target
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Function
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. -
Sequence similarities
Contains 1 WAP domain. -
Domain
Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5266 Human
- Omim: 182257 Human
- SwissProt: P19957 Human
- Unigene: 112341 Human
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Alternative names
- Cementoin antibody
- ELAF_HUMAN antibody
- Elafin antibody
see all
Images
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![Western blot - Anti-Elafin/ESI antibody [EPR14631] - BSA and Azide free (ab236135)](https://www.abcam.com/ps/products/236/ab236135/Images/ab236135-311867-236135.jpg)
Western blot - Anti-Elafin/ESI antibody [EPR14631] - BSA and Azide free (ab236135)Anti-Elafin/ESI antibody [EPR14631] (ab184972) at 1/2000 dilution + A431 cell lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184972).
Datasheets and documents
References
ab236135 has not yet been referenced specifically in any publications.
