Recombinant
RabMAb

Recombinant Anti-Elafin/ESI antibody [EPR14631] - BSA and Azide free (ab236135)

Overview

  • Product name

    Anti-Elafin/ESI antibody [EPR14631] - BSA and Azide free
    See all Elafin/ESI primary antibodies
  • Description

    Rabbit monoclonal [EPR14631] to Elafin/ESI - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Elafin/ESI aa 1-100. The exact sequence is proprietary.
    Database link: P19957

  • Positive control

    • WB: A431 cell lysate.
  • General notes

    ab236135 is the carrier-free version of ab184972 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236135 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Elafin/Skalp.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 12, 14 kDa (predicted molecular weight: 12 kDa).

Target

  • Function

    Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
  • Sequence similarities

    Contains 1 WAP domain.
  • Domain

    Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cementoin antibody
    • ELAF_HUMAN antibody
    • Elafin antibody
    • Elafin/Skalp antibody
    • Elastase Specific Inhibitor antibody
    • Elastase-specific inhibitor antibody
    • ES 1 antibody
    • ESI antibody
    • Peptidase inhibitor 3 antibody
    • Peptidase inhibitor 3, skin derived antibody
    • PI 3 antibody
    • PI-3 antibody
    • PI3 antibody
    • Pre elafin antibody
    • Protease inhibitor 3 skin derived antibody
    • Protease inhibitor 3, skin derived (SKALP) antibody
    • Protease inhibitor WAP3 antibody
    • SKALP antibody
    • Skin derived Anti leukoproteinase antibody
    • Skin-derived antileukoproteinase antibody
    • Trappin 2 antibody
    • WAP four disulfide core domain 14 antibody
    • WAP four disulfide core domain protein 14 antibody
    • WAP four-disulfide core domain protein 14 antibody
    • WAP3 antibody
    • WFDC14 antibody
    see all

Images

  • Anti-Elafin/ESI antibody [EPR14631] (ab184972) at 1/2000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184972).

References

ab236135 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236135.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up